Enzyme kinetics is the study of the
rates of
chemical reactions that are
catalysed by
enzymes. The study of an enzyme's
kinetics provides insights into the catalytic mechanism of this enzyme, its role in
metabolism, how its activity is controlled in the
cell and how
drugs and
poisons can
inhibit its activity.
Enzymes are molecules that manipulate other molecules — the enzymes' substrates. These target molecules bind to an enzyme's active site and are transformed into products through a series of steps known as the enzymatic mechanism. Some enzymes bind multiple substrates and/or release multiple products, such as a protease cleaving one protein substrate into two polypeptide products. Others join substrates together, such as DNA polymerase linking a nucleotide to DNA. Although these mechanisms are often a complex series of steps, there is typically one rate-determining step that determines the overall kinetics. This rate-determining step may be a chemical reaction or a conformational change of the enzyme or substrates, such as those involved in the release of product(s) from the enzyme.
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